缺血后处理对局灶性脑缺血大鼠的脑保护作用

作者：宫利  王艺东  王志  周海红  陶恩祥  邢诒刚

单位：中山大学附属第二医院神经科; 中山大学附属第二医院麻醉科; 中山大学附属第二医院神经科 广州;

关键词：缺血后处理  局灶性脑缺血  梗塞面积  行为学  神经保护

分类号：

出版年·卷·期（页码）：2008··第四期（205-207）

摘要：

目的探讨缺血后处理(ischemic postconditioning,IP)对局灶性脑缺血大鼠的脑保护作用。方法36只SD大鼠采用开颅机械闭塞法建立局灶性脑缺血模型后,随机分为2组(n=18),IP组:阻断大脑中动脉(middle cerebral artery,MCA)15min后开放双侧的颈总动脉(bilateral common carotid arteries,bCCAs)15s,夹闭15s,反复3次,然后开放bCCAs进行永久性灌注;非IP组:阻断MCA15min后直接开放bCCAs进行永久性灌注。分别于术前1d,术后30d内在各时间点行行为学检测,并以正常大鼠为空白对照。于再灌后2d、14d时各组随机取6只检测脑梗面积。结果IP组大鼠在各时间点行为学检测结果均优于非IP组;再灌注2d、14d时IP组脑梗面积均显著小于非IP组(P<0.001),IP组再灌注2d和14d的脑梗面积无统计学差异(P=0.724)。结论IP可改善局灶性脑缺血大鼠的神经功能,降低脑梗面积。

Objective To investigate the neuroprotection of ischemic postconditioning (IP ) against reperfusion injury in a rat mode of focal cerebral ischemia model. Methods Focal ischemia was generated by permanent distal middle cerebral artery (MCA) occlusion plus transient bilateral common carotid artery occlusion (bCCAs). Ischmeic animals were randomly assigned to 2 groups (n=18): IP group and non-IP group. In IP group, animals with MCAO were further subject to postconditioning (3 cycles of 15 s ischemia and 15 s ...

参考文献：

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