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阿尔茨海默病患者外周血巨噬细胞移动抑制因子的表达
作者: 韩冰莎 王丽娟 赵洁皓 黄智恒 马桂贤 HAN Bingsha WANG Lijuan ZHAO Jiehao HUANG Zhiheng MA Guixian. Department of Neurology Guangdong Provincial People s Hospital Guangzhou 510515.
单位:南方医科大学; 广东省人民医院神经内科; 广东省人民医院神经内科 在读硕士研究生;
关键词: 阿尔茨海默病 巨噬细胞移动抑制因子 荧光定量PCR
分类号:
出版年·卷·期(页码):2008·34·第 4 期(0-0)
摘要:
目的探讨阿尔茨海默病(Alzheimer's disease,AD)患者外周血中巨噬细胞移动抑制因子(macro-phage migration inhibitory factor,MIF)的表达水平及其与病情程度的关系。方法应用ELISA法和荧光定量PCR技术,测定64例AD患者和50名健康对照者外周血MIF的表达水平。根据简易精神状况检查量表(MMSE)评分将AD患者分为轻、中、重度3组。结果血浆MIF的浓度和外周血单个核细胞(peripheral blood mononuclear cells,PB-MC)MIF mRNA的表达水平:AD组高于对照组,差别有统计学意义(P0.05)。蛋白和核酸两个水平MIF的浓度与MMSE评分成负相关(r蛋白=-0.34,r核酸=-0.36,P<0.01)。结论MIF可能参与AD的病理过程并发挥重要作用,其水平变化与痴呆严重程度相关。
Objective To investigate the expression of macrophage migration inhibitory factor(MIF)in peripheral blood mononuclear cells (PBMC) of patients with Alzheimer s disease (AD), and its correlation with dementia severity. Methods The levels of MIF protein and mRNA in PBMC from 64 AD patients and 50 healthy subjects were measured using ELISA and Fluorogenic quantitative PCR, respectively. AD patients were classified into 3 groups according to MMSE scores as follows: mild, moderate and severe group. Results The e...
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西文参考文献 共找到 9 条
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