人脑转移癌组织裸小鼠脑内移植模型的建立-中国神经精神疾病杂志

人脑转移癌组织裸小鼠脑内移植模型的建立

单位：苏州大学附属第二医院神经外科; 苏州大学附属第二医院神经外科苏州;

分类号：

出版年·卷·期（页码）：2008··第四期（216-220）

摘要：

目的建立人肺腺癌脑转移瘤的裸小鼠原位移植模型,并分析移植瘤的生物学特征。方法取人肺腺癌脑转移瘤组织块,接种于裸小鼠右尾状核。致瘤后即用裸小鼠的瘤组织进行其原位传代接种,观察致瘤率及荷瘤鼠生存期;MRI观察移植瘤在鼠脑内的大体形态;HE染色分析各代移植瘤的组织学形态;免疫组化染色观察移植瘤中CEA的表达;Alcian blue/PAS特殊染色检测移植瘤中粘液的性质。结果移植瘤组织在裸小鼠右尾状核已连续传至6代,共65只鼠。荷瘤鼠生存期:原代为(47.6±1.8)d,2~3代有所缩短,4~6代稳定在(38.0±0.9)d;移植瘤MRI类圆形,瘤周无水肿。移植瘤病理为低分化腺癌,不向周围正常鼠脑组织浸润;移植瘤中CEA表达阳性,有酸性粘液存在。结论人肺腺癌脑转移瘤组织块接种于鼠脑内建立的原位移植模型能更好地模拟临床原发肿瘤的病理学特征,本方法为研究人肺腺癌脑转移瘤的生物学特性及实验治疗提供了一个可靠的动物模型。

Objective To establish an orthotopic transplantation model of brain metastasis of human lung adenocarcinoma in nude mice and analyze its biological features.Methods Human brain metastatic tumor masses were collected and orthotopicly transplanted into the right caudate nucleus of nude mice. The newly formed xenografts were sharply minced into pieces and re-transplanted. The efficiency of tumorigenicity and the survival time of tumor-bearing mice were observed. HE staining and MRI scanning were performed to i...

参考文献：

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