阻断ERK通路的再激活对吗啡依赖大鼠已建立的条件性位置偏爱的影响

作者：邵云  韩继阳  何强  王旭梅

单位：中国医科大学附属盛京医院心理门诊; 中国医科大学附属盛京医院心理门诊 沈阳;

关键词：吗啡  依赖  条件性位置偏爱  细胞外信号调节激酶  伏隔核

分类号：

出版年·卷·期（页码）：2008··第一期（31-34）

摘要：

目的研究阻断细胞外信号调节激酶(ERK)通路的再激活对吗啡依赖大鼠已建立的条件性位置偏爱(CPP)的影响。方法将80只大鼠随机分成8组每组各10只,Ⅱ-Ⅷ组为实验组,Ⅰ组为对照组。在建立吗啡的CPP后,分别给予腹腔注射细胞外信号调节激酶抑制剂SL327或生理盐水后再次注射吗啡,对照组仅注射生理盐水,并与给药环境建立联系,观察对已建立的CPP的影响。并采用western blotting法测定伏隔核中磷酸化ERK1/2(P-ERK1/2)的表达水平。结果①CPP测试前各组在白箱内(伴药侧)停留的时间差异无统计学意义(P>0.05)。②经过6d条件反射训练,各实验组大鼠在白箱内停留的时间明显比对照组长,差异均有统计学意义(P<0.01),表明实验组大鼠均已建立起对白盒的CPP。③给予已建立CPP的各实验组大鼠不同处置,结果表明,只有给予腹腔注射SL327后再次注射吗啡的Ⅳ组大鼠的CPP已消除,该组在白箱内的停留时间较其它实验组有显著性差异(P<0.01),而与对照组的差异无统计学意义(P>0.05)。同时,Ⅳ组大鼠伏隔核中P-ERK1/2的表达水平显著低于其它实验组(P<0.01),但其与对照组间的差异无统计学意...

Objective To study the influence of blockade of ERK pathway reactivation on the previously learned place preference in the morphine-addicted rats.Methods Eighty rats were randomly assigned into eight groups with ten in each group:the first group was the control group,and the other seven groups were the experimental groups.After the establishment of morphine-producing conditioned place preference(CPP),the rats in the experimental groups were administered peritoneal injection of morphine after normal saline o...

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